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Southern blot | Biomolecules | MCAT | Khan Academy

Оценок: 932 | Просмотров: 220897
Visit us (http://www.khanacademy.org/science/healthcare-and-medicine) for health and medicine content or (http://www.khanacademy.org/test-prep/mcat) for MCAT related content. These videos do not provide medical advice and are for informational purposes only. The videos are not intended to be a substitute for professional medical advice, diagnosis or treatment. Always seek the advice of a qualified health provider with any questions you may have regarding a medical condition. Never disregard professional medical advice or delay in seeking it because of something you have read or seen in any Khan Academy video. Created by Ronald Sahyouni. Watch the next lesson: https://www.khanacademy.org/test-prep/mcat/biomolecules/dna-technology/v/southern-blot?utm_source=YT&utm_medium=Desc&utm_campaign=mcat Missed the previous lesson? https://www.khanacademy.org/test-prep/mcat/biomolecules/dna-technology/v/hybridization-microarray?utm_source=YT&utm_medium=Desc&utm_campaign=mcat MCAT on Khan Academy: Go ahead and practice some passage-based questions! About Khan Academy: Khan Academy offers practice exercises, instructional videos, and a personalized learning dashboard that empower learners to study at their own pace in and outside of the classroom. We tackle math, science, computer programming, history, art history, economics, and more. Our math missions guide learners from kindergarten to calculus using state-of-the-art, adaptive technology that identifies strengths and learning gaps. We've also partnered with institutions like NASA, The Museum of Modern Art, The California Academy of Sciences, and MIT to offer specialized content. For free. For everyone. Forever. #YouCanLearnAnything Subscribe to Khan Academy’s MCAT channel: https://www.youtube.com/channel/UCDkK5wqSuwDlJ3_nl3rgdiQ?sub_confirmation=1 Subscribe to Khan Academy: https://www.youtube.com/subscription_center?add_user=khanacademy
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Текстовые комментарии (41)
khizre hussain (3 месяца назад)
Best video on u tube for southern blotting...thanks a lot
ATISH MOHAPATRA (5 месяцев назад)
Simplified and the best video lecture on southern blotting .
sumaiya islam (8 месяцев назад)
veey well explained ..thanku sir
Tuan Ngoc Nguyen (10 месяцев назад)
great explanation. love your channel.
khalida02 (1 год назад)
he didn't mention that #3 is referred to as the hybridization step
Johannes Mthembu (1 год назад)
Thank you well explained!!!
Di Hu (1 год назад)
better than my professor's PPT
Maria Paula Pedro Morgado (1 год назад)
Amazing! Thank you so much
崔雍建 (1 год назад)
what if it happens that the gene is cut into different fragments which are located in different parts on the gel? Will radiation be detected in several spots then or none of the spots?
Wesley Richardson (1 год назад)
Not a very concise explanation is it?
Mohit Gangrade (1 год назад)
nice one @khanacademy. but u missed the NaOH step which is important for denaturing. besides that it was great
Mohammad O. Eshkalak (1 год назад)
These guy is wrong all over. He has just learnt about Southern Blot probably. Khan academy must Rethink on those selected for teaching these. I believe they are geetting paid good money so please present high quality stuff.
Swordsman0 (1 год назад)
No. I'm Good
Alex Harper (1 год назад)
genie.
Nur (2 года назад)
what is the aspect difference between the three method ? northern, southern and western . i only find a few differences bethween them ? Can you identify the difference>
Lia (1 год назад)
basically , Southern is used to detect DNA ,Northern is for RNA and Western for polypeptides.
BlackLukeS (2 года назад)
Very very helpful. Thank you
Reem Shwan (2 года назад)
I wanna cry :') that was very helpful thanks I love you
saxeli gvari (2 года назад)
Yes but you missed out one important part. NaOH is added to those double stranded DNA fragments to denature them, otherwise complementary probe wont anneal to the target sequence
Jessica Yakobashvili (7 месяцев назад)
madlobt :)
Max Weber (11 месяцев назад)
Ü
Abid Abdelaziz (1 год назад)
exactly what i was thinking :D !
Nadine Puti-an (1 год назад)
saxeli gvari thank you!! i was a bit confused but thanks for clearing it up :)
Uzithobile Sifuba (2 года назад)
Thanks , this is so helpful
Wayne (2 года назад)
what if the enzymes cut the gene a in half so the complementary dna can not completely allign?
Rafa Juerto (2 года назад)
+Wayne The enzymes will cut at a specific sequence of nucleotides every time. You will select the enzymes you want to use based on the sequence of your gene and you can ensure that the enzymes will cut in the correct place. It is possible that the enzymes will make a mistake but this won't be very often so the majority of the product will be of the correct length.
Nao Tomori (2 года назад)
are piece of DNA=DNA fragments also ds? if yes, how radio-labeled DNA anneals o.O?
Rafa Juerto (2 года назад)
+Nao Tomori You denature the dna in the gel by soaking the entire gel in NaOH before transferring it to the membrane which makes the DNA single stranded. The probe is also single stranded.
Ventsi24 (2 года назад)
+Nao Tomori It has complimentary bases to the DNA fragment so forms hydrogen bonds with it
rima yousuf (2 года назад)
thank youuuuu
Belle Fille (2 года назад)
Really helpful video. Thanks!
Aaron (2 года назад)
Full of left out steps and mistakes. Be careful.
Aaron (2 года назад)
+Aaron You can see the video on khanacademy, I believe I listed some of the problems there.
Aaron (2 года назад)
+Mahela Munasinghe I'll have to come back here and answer you after my Population and Community Eco final. :)
Mahela Munasinghe (2 года назад)
+Aaron what are the mistakes?
Heng Ju Lin (3 года назад)
tks~~helpful~~
Nalley Adam (3 года назад)
very well explained :) thx buddy
Brian Tran (3 года назад)
how does the DNA probe anneal to the the fragments? after restriction enzymes cut are they single stranded?
Aruna Boro (11 месяцев назад)
the probe used is complementary to the targetted gene
hoa nguyen (1 год назад)
I couldnt agree with you more, this is a hibridization in which single stranded cDNA/ RNA will bind to another DNA/ RNA (the one we want to detect) in order to form a hibrid
xGodzAssassin (3 года назад)
Yes the gel is treated with alkali that separates the 2 DNA strands into single strands

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